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KMID : 0378019700130030108
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New Medical Journal
1970 Volume.13 No. 3 p.108 ~ p.114
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A study on the macrophage-staphylococcus interactions in vitro
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Abstract
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Numerous studies on the interactions between phagocytes and microorganisms were reported; however, there remain many aspects to be investigated. The phagocytosis and the fate of microorganisms may be influenced by the various factors such as the metabolism of the phagocytic cells, the nature of the bacterial surface substances, the physico-chemical factors of the environments, etc.
It is well known that the staphylococcal infections are essentially intracellular infections and that the pathogenicities of Staph aureus and Staph. albus are quite different. It is also known that the metabolic activities of various cells may be influenced by the change of environmental temperature. A
Thus, an experiment was planned and performed to study the mode of interactions betweqh rabbit macrophages and Staph. aureus or Staph. albus at different temperatures.
The growth patterns of Staph. aureus and Staph. albus in the cell maintenance fluid (CMF) medium were followed for 5 hours at 37°C. and 33°C. Normal rabbit macrophages were collected from the peritoneal cavity, suspended in the the CMF medium and mixed with the suspension of staphylococci. The macrophage-bacteria suspensions were maintained for 5 hours at 37°C. and 330C., and the rate of the appearance of the macrophages containing the organisms, and the extracellular and intracellular viable populations of the organisms were studied.
The results of the observation were summerazied as follow:
1) Staph. aureus began to increase, at 37°C. and 33C., more rapidly and actively than Staph. albus in the CMF medium.
2) The macrophages containing Staph. albus appeared more rapidly than the macrophages containing Staph. aureus.
3) The intra-and extra-cellular viable units of Staph. aureus showed more or less increase, and the percentage of the intracelluar units to the total viable units increased upto 3 hours and decreased thereafter.
4) The intra-and extra-cellular viable units of Staph. albus also increased, but the increase of the intracellular units was more sluggish than the extracellular units. The percentage of the intracellular units to the total units decreased after I hour.
No significant difference was noticed between the interactions of the macrophage-bacteria at 37°C. and at 33°C.
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